GSM6421758: Ishikawa 1hrE2 rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ESR1
Cell type
Cell type Class
Uterus
Cell type
Ishikawa
Primary Tissue
Uterus
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Ishikawa
cell line
Ishikawa
cell type
endometrioid adenocarcinoma
chip antibody
ESR1 (Millipore Sigma, 06-935)
treatment
10 nM E2
Sequenced DNA Library
library_name
GSM6421758
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed using 1% formaldehyde (Sigma Aldrich, 252549) for 10 minutes at room temperature to cross-link DNA and proteins. Cross-linking was stopped by addition of glycine (Fisher Scientific, BP381) to a final concentration of 125 mM. Cells were then washed with cold PBS and harvested via scraping in Farnham lysis buffer supplemented with protease and phosphatase inhibitors (Thermo Scientific, A32959). Chromatin immunoprecipitation was performed as previously described (Reddy et al. Genome Research 2009) with an Anti-Estrogen receptor alpha (ESR1) antibody (Millipore Sigma, 06-935) or H3K27ac antibody (Active Motif, 39133).